A modified Langmuir model for the prediction of the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents

Q. Lan; A. S. Bassi; J. X. Zhu; A. Margaritis

doi:10.1016/S1385-8947(00)00197-2

A modified Langmuir model for the prediction of the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents

Q. Lan, A. S. Bassi, J. X. Zhu, A. Margaritis

Research output: Journal Publication › Article › peer-review

132 Citations (Scopus)

Abstract

An empirical model modified from the Langmuir isotherm model to account for the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents has been proposed and tested against experimental and literature data. The equilibrium isotherms for BSA adsorption onto a polystyrenic anion exchanger, Diaion HPA25, were established for five different NaCl concentrations at 25°C, pH 7.0. The apparent Langmuir parameters in the new model (q′_m and K′_d), which replace the Langmuir parameters (q_m and K_d) in the original Langmuir model, were determined by non-linear curve fitting. The proposed model has been shown to be applicable to various protein/adsorbent systems.

Original language	English
Pages (from-to)	179-186
Number of pages	8
Journal	Chemical Engineering Journal
Volume	81
Issue number	1-3
DOIs	https://doi.org/10.1016/S1385-8947(00)00197-2
Publication status	Published - 1 Jan 2001
Externally published	Yes

Keywords

Ion exchange
Ionic strength
Langmuir isotherm
Modeling
Protein adsorption

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry
General Chemical Engineering
Industrial and Manufacturing Engineering

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10.1016/S1385-8947(00)00197-2

Cite this

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title = "A modified Langmuir model for the prediction of the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents",

abstract = "An empirical model modified from the Langmuir isotherm model to account for the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents has been proposed and tested against experimental and literature data. The equilibrium isotherms for BSA adsorption onto a polystyrenic anion exchanger, Diaion HPA25, were established for five different NaCl concentrations at 25°C, pH 7.0. The apparent Langmuir parameters in the new model (q′m and K′d), which replace the Langmuir parameters (qm and Kd) in the original Langmuir model, were determined by non-linear curve fitting. The proposed model has been shown to be applicable to various protein/adsorbent systems.",

keywords = "Ion exchange, Ionic strength, Langmuir isotherm, Modeling, Protein adsorption",

author = "Q. Lan and Bassi, {A. S.} and Zhu, {J. X.} and A. Margaritis",

note = "Funding Information: Financial support from the Natural Sciences and Engineering Research Council of Canada Strategic Grant is gratefully acknowledged. ",

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doi = "10.1016/S1385-8947(00)00197-2",

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T1 - A modified Langmuir model for the prediction of the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents

AU - Lan, Q.

AU - Bassi, A. S.

AU - Zhu, J. X.

AU - Margaritis, A.

N1 - Funding Information: Financial support from the Natural Sciences and Engineering Research Council of Canada Strategic Grant is gratefully acknowledged.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - An empirical model modified from the Langmuir isotherm model to account for the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents has been proposed and tested against experimental and literature data. The equilibrium isotherms for BSA adsorption onto a polystyrenic anion exchanger, Diaion HPA25, were established for five different NaCl concentrations at 25°C, pH 7.0. The apparent Langmuir parameters in the new model (q′m and K′d), which replace the Langmuir parameters (qm and Kd) in the original Langmuir model, were determined by non-linear curve fitting. The proposed model has been shown to be applicable to various protein/adsorbent systems.

AB - An empirical model modified from the Langmuir isotherm model to account for the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents has been proposed and tested against experimental and literature data. The equilibrium isotherms for BSA adsorption onto a polystyrenic anion exchanger, Diaion HPA25, were established for five different NaCl concentrations at 25°C, pH 7.0. The apparent Langmuir parameters in the new model (q′m and K′d), which replace the Langmuir parameters (qm and Kd) in the original Langmuir model, were determined by non-linear curve fitting. The proposed model has been shown to be applicable to various protein/adsorbent systems.

KW - Ion exchange

KW - Ionic strength

KW - Langmuir isotherm

KW - Modeling

KW - Protein adsorption

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VL - 81

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A modified Langmuir model for the prediction of the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents

Abstract

Keywords

ASJC Scopus subject areas

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