Effects of Lipids and Type of Amino Acid in Protein in Microalgae on Nitrogen Reaction Pathways during Hydrothermal Liquefaction

It is meaningful to understand the conversion pathways of nitrogen during the hydrothermal liquefaction process of microalgae to reveal the related reaction mechanisms and develop effective methods to prevent N from ending in biocrude, which eventually increases the quality of biocrude. Extending from our previous works that mainly focused on two high-protein (>50 wt%) microalgae (Chlorella sp. and Spirulina sp.), Nannochloropsis sp., which has a high lipid content (>70 wt%), was used as the feedstock for this project using the same methodology. The high lipid content in Na. induced less nitrogen during the oil phase and as a result, reduced the heteroatom content while also improving the quality of biocrude. It is worth noting that another investigation was conducted on the model compounds with different types of amino acids to specify the effects of the types of amino acids in the proteins in microalgae on the N pathway and their distribution in the products (aqueous phase, oil, solid, and gas). It was found that the basic amino acid in microalgae caused the formation of more N-heterocyclic compounds in the biocrude. The mass flow based on the mass balance was demonstrated to further refine the map showing the predicted reaction pathway of nitrogen from the previous version.