Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination

Pei Cao; Nasim Amiralian; Jingjing Wang; Bing Sun; Amirali Popat; Fengwei Xie; Zhi Ping Xu; Yan Li; Li Li

doi:10.1016/j.bioadv.2023.213400

Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination

Pei Cao, Nasim Amiralian, Jingjing Wang, Bing Sun, Amirali Popat, Fengwei Xie, Zhi Ping Xu, Yan Li, Li Li

Research output: Journal Publication › Article › peer-review

2 Citations (Scopus)

Abstract

Oral vaccine is a non-invasive, ideal way to protect communities from infectious diseases. Effective vaccine delivery systems are required to enhance vaccine absorption in the small intestine and its cellular uptake by immune cells. Here, we constructed alginate/chitosan-coated cellulose nanocrystal (Alg-Chi-CNC) and nanofibril (Alg-Chi-CNF) nanocomposites to enhance ovalbumin (OVA) delivery in the intestine. In vitro mucosal permeation and diffusion and cellular uptake demonstrated that Chi-CNC exhibited better cellular uptake in epithelial and antigen-presenting cells (APCs). In vivo results revealed that alginate/chitosan-coated nanocellulose nanocomposites generated strong systemic and mucosal immune responses. Though the features of functional nano-cellulose composites affected mucus permeation and APC uptakes, in vivo specific-OVA immune responses have not shown significant differences due to the complexity of the small intestine.

Original language	English
Article number	213400
Journal	Biomaterials Advances
Volume	149
DOIs	https://doi.org/10.1016/j.bioadv.2023.213400
Publication status	Published - Jun 2023
Externally published	Yes

Keywords

Antigen internalization
Nano-cellulose composites
Oral vaccine delivery
Particle size
Polysaccharides
Stiffness

ASJC Scopus subject areas

Bioengineering
Biomaterials
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bioadv.2023.213400

Cite this

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title = "Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination",

abstract = "Oral vaccine is a non-invasive, ideal way to protect communities from infectious diseases. Effective vaccine delivery systems are required to enhance vaccine absorption in the small intestine and its cellular uptake by immune cells. Here, we constructed alginate/chitosan-coated cellulose nanocrystal (Alg-Chi-CNC) and nanofibril (Alg-Chi-CNF) nanocomposites to enhance ovalbumin (OVA) delivery in the intestine. In vitro mucosal permeation and diffusion and cellular uptake demonstrated that Chi-CNC exhibited better cellular uptake in epithelial and antigen-presenting cells (APCs). In vivo results revealed that alginate/chitosan-coated nanocellulose nanocomposites generated strong systemic and mucosal immune responses. Though the features of functional nano-cellulose composites affected mucus permeation and APC uptakes, in vivo specific-OVA immune responses have not shown significant differences due to the complexity of the small intestine.",

keywords = "Antigen internalization, Nano-cellulose composites, Oral vaccine delivery, Particle size, Polysaccharides, Stiffness",

author = "Pei Cao and Nasim Amiralian and Jingjing Wang and Bing Sun and Amirali Popat and Fengwei Xie and Xu, {Zhi Ping} and Yan Li and Li Li",

note = "Publisher Copyright: {\textcopyright} 2023",

year = "2023",

month = jun,

doi = "10.1016/j.bioadv.2023.213400",

language = "English",

volume = "149",

journal = "Biomaterials Advances",

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T1 - Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination

AU - Cao, Pei

AU - Amiralian, Nasim

AU - Wang, Jingjing

AU - Sun, Bing

AU - Popat, Amirali

AU - Xie, Fengwei

AU - Xu, Zhi Ping

AU - Li, Yan

AU - Li, Li

PY - 2023/6

Y1 - 2023/6

N2 - Oral vaccine is a non-invasive, ideal way to protect communities from infectious diseases. Effective vaccine delivery systems are required to enhance vaccine absorption in the small intestine and its cellular uptake by immune cells. Here, we constructed alginate/chitosan-coated cellulose nanocrystal (Alg-Chi-CNC) and nanofibril (Alg-Chi-CNF) nanocomposites to enhance ovalbumin (OVA) delivery in the intestine. In vitro mucosal permeation and diffusion and cellular uptake demonstrated that Chi-CNC exhibited better cellular uptake in epithelial and antigen-presenting cells (APCs). In vivo results revealed that alginate/chitosan-coated nanocellulose nanocomposites generated strong systemic and mucosal immune responses. Though the features of functional nano-cellulose composites affected mucus permeation and APC uptakes, in vivo specific-OVA immune responses have not shown significant differences due to the complexity of the small intestine.

AB - Oral vaccine is a non-invasive, ideal way to protect communities from infectious diseases. Effective vaccine delivery systems are required to enhance vaccine absorption in the small intestine and its cellular uptake by immune cells. Here, we constructed alginate/chitosan-coated cellulose nanocrystal (Alg-Chi-CNC) and nanofibril (Alg-Chi-CNF) nanocomposites to enhance ovalbumin (OVA) delivery in the intestine. In vitro mucosal permeation and diffusion and cellular uptake demonstrated that Chi-CNC exhibited better cellular uptake in epithelial and antigen-presenting cells (APCs). In vivo results revealed that alginate/chitosan-coated nanocellulose nanocomposites generated strong systemic and mucosal immune responses. Though the features of functional nano-cellulose composites affected mucus permeation and APC uptakes, in vivo specific-OVA immune responses have not shown significant differences due to the complexity of the small intestine.

KW - Antigen internalization

KW - Nano-cellulose composites

KW - Oral vaccine delivery

KW - Particle size

KW - Polysaccharides

KW - Stiffness

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DO - 10.1016/j.bioadv.2023.213400

M3 - Article

AN - SCOPUS:85151460768

SN - 2772-9508

VL - 149

JO - Biomaterials Advances

JF - Biomaterials Advances

M1 - 213400

ER -

Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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