Evaluation of dynamic binding performance of C-phycocyanin and allophycocyanin in Spirulina platensis algae by aminated polyacrylonitrile nanofiber membrane

Polyacrylonitrile nanofibrous membrane (PAN) was prepared by electrospinning technique and was directly converted to polyamine membrane by 50 % and 100 % (w/w) ethylenediamine (EDA) and hexamethylenediamine (HDA), respectively. The aminated PAN nanofiber membrane equipped with protein liquid chromatography were used to evaluate the dynamic binding capacity for C-phycocyanin (CPC) and allophycocyanin (APC) from disrupted Spirulina platensis under different operating conditions. Experimental results showed that the contaminating proteins were completely removed during the flow and wash procedures. However, a large amount of APC and CPC can be retained on the membranes. Consequently, the aminated PAN membrane may have a high potential for use in the separation of APC/CPC and TP from a complex algae feedstock. In this study, a highly effective capture of APC/CPC can be achieved in one-step membrane chromatography, which can be directly employed as an antibacterial and antioxidant nanofiber membranes.