Fabrication and Evaluation of Hyaluronidase-Responsive Scaffolds by Electrospinning with Antibacterial Properties for Tympanic Membrane Repair

Zhechen Yuan, Bing Mei Teh, Xiaoling Liu, Ziqian Liu, Juntao Huang, Yi Hu, Chengchen Guo, Yi Shen

Research output: Journal PublicationArticlepeer-review

Abstract

Tympanic membrane perforation (TMP) is prevalent in clinical settings. Patients with TMPs often suffer from infections caused by Staphylococcus aureus and Pseudomonas aeruginosa, leading to middle ear and external ear canal infections, which hinder eardrum healing. The objective of this study is to fabricate an enzyme-responsive antibacterial electrospun scaffold using poly(lactic-co-glycolic acid) and hyaluronic acid for the treatment of infected TMPs. The properties of the scaffold were characterized, including morphology, wettability, mechanical properties, degradation properties, antimicrobial properties, and biocompatibility. The results indicated that the fabricated scaffold had a core-shell structure and exhibited excellent mechanical properties, hydrophobicity, degradability, and cytocompatibility. Furthermore, in vitro bacterial tests and ex vivo investigations on eardrum infections suggested that this scaffold possesses hyaluronidase-responsive antibacterial properties. It may rapidly release antibiotics when exposed to the enzyme released by S. aureus and P. aeruginosa. These findings suggest that the scaffold has great potential for repairing TMPs with infections.

Original languageEnglish
Pages (from-to)4400-4410
Number of pages11
JournalACS Biomaterials Science and Engineering
Volume10
Issue number7
DOIs
Publication statusPublished - 8 Jul 2024

Keywords

  • antibacterial
  • electrospinning
  • hyaluronidase-responsive
  • repair
  • tissue engineering
  • tympanic membrane perforation

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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